Sydney S. Choi – Life Science Abstract This study explores the efficacy of 254 nm UV-C light in sterilizing water samples from notable bodies of water in Vancouver, including Burnaby Lake, False Creek, and the Science World Wetlands. UV-C light, with its shortest wavelength among UV types (200-280 nm), is known for its strong sterilization […]
Exploring Off-Target Effects of CRISPR-Cas9 on Escherichia coli DH5α: Impact of lacZ Gene Removal on Antibiotic Sensitivity
Karina Freire Kloecking – Life Science Abstract This study aimed to investigate the off-target effects of CRISPR-Cas9 on Escherichia coli (E. coli) DH5α, focusing on the impact of the lacZ gene removal on antibiotic sensitivity and shedding light on CRISPR’s broader implications. Using CRISPR technology and a miniPCR “Knockout! A CRISPR/Cas Gene Targeting Lab” kit, […]
Evaluating the effect of Acid Rain on the Concentration of Gram-Negative Bacteria within the Rhizosphere of Brassica oleracea Sprouts
Ella Xie – Life Science Abstract The purpose of this experiment was to distinguish the relationship between soil acidity and degradation of a plant’s rhizosphere. The concentration of gram-negative bacteria was observed as soil bacteria in the rhizosphere is mainly gram-negative. Individual containers of broccoli sprouts Brassica oleracea were exposed to different pH levels of […]
The Challenges and Lessons Inside a Hexapod Robot Prototype
Michael Hewko, Year 2 Engineering Abstract Legged locomotion applied to robots or vehicles is a topic of widespread interest, study and application. A hexapod (six-legged) robot was created using the Arduino platform to carry weight and cross complex terrain. The robot failed to walk due to desynchronization between servo motors and commands. Suggested fixes to […]